Construct: shRNA TRCN0000039389

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010411.1-1145s1c1
DNA Barcode:
CGTGGCTCTCTGAAACCTTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Hdac3 (15183)
Gene Description:
histone deacetylase 3
Transcript:
RefSeq NM_010411.1 (NON-CURRENT)
Match location:
Position 1145 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000039389)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTGGCTCTCTGAAACCTTAA
Hairpin Sequence:
5'-CCGG-CGTGGCTCTCTGAAACCTTAA-CTCGAG-TTAAGGTTTCAGAGAGCCACG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTGGCTCTCTGAAACCTTAACTCGAGTTAAGGTTTCAGAGAGCCACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTGGCTCTCTGAAACCTTAACTCGAGTTAAGGTTTCAGAGAGCCACG-3'

Other clones with same target sequence:

  1. TRCN0000318154