Construct: shRNA TRCN0000039456

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_033604.1-1385s1c1
DNA Barcode:
GCCAAGGTTCAAGTTCGCATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rnf111 (93836)
Gene Description:
ring finger 111
Transcript:
RefSeq NM_033604.1 (NON-CURRENT)
Match location:
Position 1385 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000039456)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAAGGTTCAAGTTCGCATA
Hairpin Sequence:
5'-CCGG-GCCAAGGTTCAAGTTCGCATA-CTCGAG-TATGCGAACTTGAACCTTGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAAGGTTCAAGTTCGCATACTCGAGTATGCGAACTTGAACCTTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAAGGTTCAAGTTCGCATACTCGAGTATGCGAACTTGAACCTTGGC-3'

Other clones with same target sequence:

  1. TRCN0000287658