Construct: shRNA TRCN0000039459

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145538.1-894s1c1
DNA Barcode:
CCAAACCTGAACTGAAGCCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tmem189 (407243)
Gene Description:
transmembrane protein 189
Transcript:
RefSeq NM_145538.1 (NON-CURRENT)
Match location:
Position 894 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000039459)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAAACCTGAACTGAAGCCAT
Hairpin Sequence:
5'-CCGG-CCAAACCTGAACTGAAGCCAT-CTCGAG-ATGGCTTCAGTTCAGGTTTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAAACCTGAACTGAAGCCATCTCGAGATGGCTTCAGTTCAGGTTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAAACCTGAACTGAAGCCATCTCGAGATGGCTTCAGTTCAGGTTTGG-3'

Other clones with same target sequence:

  1. TRCN0000324023