Construct: shRNA TRCN0000039472

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025356.2-519s1c1
DNA Barcode:
CTCCTGCTTTAACTATTTCTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ube2d3 (66105)
Gene Description:
ubiquitin-conjugating enzyme E2D 3
Transcript:
RefSeq NM_025356.2 (NON-CURRENT)
Match location:
Position 519 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000039472)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCCTGCTTTAACTATTTCTA
Hairpin Sequence:
5'-CCGG-CTCCTGCTTTAACTATTTCTA-CTCGAG-TAGAAATAGTTAAAGCAGGAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCCTGCTTTAACTATTTCTACTCGAGTAGAAATAGTTAAAGCAGGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCCTGCTTTAACTATTTCTACTCGAGTAGAAATAGTTAAAGCAGGAG-3'

Other clones with same target sequence:

(none)