Construct: shRNA TRCN0000039529

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_053170.1-2610s1c1
DNA Barcode:
CGTCTGTTACAGCAATAGAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Trim33 (94093)
Gene Description:
tripartite motif-containing 33
Transcript:
RefSeq NM_053170.1 (NON-CURRENT)
Match location:
Position 2610 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000039529)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTCTGTTACAGCAATAGAAT
Hairpin Sequence:
5'-CCGG-CGTCTGTTACAGCAATAGAAT-CTCGAG-ATTCTATTGCTGTAACAGACG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTCTGTTACAGCAATAGAATCTCGAGATTCTATTGCTGTAACAGACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTCTGTTACAGCAATAGAATCTCGAGATTCTATTGCTGTAACAGACG-3'

Other clones with same target sequence:

(none)