Construct: shRNA TRCN0000039572

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005758.1-801s1c1
DNA Barcode:
AGGTAGTTATGGAGGAGGTGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
HNRNPA3P1 (10151)
Gene Description:
heterogeneous nuclear ribonucleoprotein A3 pseudogene 1
Transcript:
RefSeq NM_005758.1 (NON-CURRENT)
Match location:
Position 801 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000039572)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGGTAGTTATGGAGGAGGTGA
Hairpin Sequence:
5'-CCGG-AGGTAGTTATGGAGGAGGTGA-CTCGAG-TCACCTCCTCCATAACTACCT-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGGTAGTTATGGAGGAGGTGACTCGAGTCACCTCCTCCATAACTACCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGGTAGTTATGGAGGAGGTGACTCGAGTCACCTCCTCCATAACTACCT-3'

Other clones with same target sequence:

(none)