Construct: shRNA TRCN0000039577

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002608.1-1100s1c1
DNA Barcode:
CGAGGAGCTTTATGAGATGCT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PDGFB (5155)
Gene Description:
platelet derived growth factor subunit B
Transcript:
RefSeq NM_002608.1 (NON-CURRENT)
Match location:
Position 1100 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000039577)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGAGGAGCTTTATGAGATGCT
Hairpin Sequence:
5'-CCGG-CGAGGAGCTTTATGAGATGCT-CTCGAG-AGCATCTCATAAAGCTCCTCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGAGGAGCTTTATGAGATGCTCTCGAGAGCATCTCATAAAGCTCCTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGAGGAGCTTTATGAGATGCTCTCGAGAGCATCTCATAAAGCTCCTCG-3'

Other clones with same target sequence:

  1. TRCN0000298819