Construct: shRNA TRCN0000039614

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001184.2-231s1c1
DNA Barcode:
CCGGATACTTACAGATGTAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ATR (545)
Gene Description:
ATR serine/threonine kinase
Transcript:
RefSeq NM_001184.2 (NON-CURRENT)
Match location:
Position 231 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000039614)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGGATACTTACAGATGTAAA
Hairpin Sequence:
5'-CCGG-CCGGATACTTACAGATGTAAA-CTCGAG-TTTACATCTGTAAGTATCCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGGATACTTACAGATGTAAACTCGAGTTTACATCTGTAAGTATCCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGGATACTTACAGATGTAAACTCGAGTTTACATCTGTAAGTATCCGG-3'

Other clones with same target sequence:

  1. TRCN0000332960