Construct: shRNA TRCN0000039713

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000267.1-8750s1c1
DNA Barcode:
CCATGTTGTAATGCTGCACTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NF1 (4763)
Gene Description:
neurofibromin 1
Transcript:
RefSeq NM_000267.1 (NON-CURRENT)
Match location:
Position 8750 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000039713)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATGTTGTAATGCTGCACTT
Hairpin Sequence:
5'-CCGG-CCATGTTGTAATGCTGCACTT-CTCGAG-AAGTGCAGCATTACAACATGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATGTTGTAATGCTGCACTTCTCGAGAAGTGCAGCATTACAACATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATGTTGTAATGCTGCACTTCTCGAGAAGTGCAGCATTACAACATGG-3'

Other clones with same target sequence:

(none)