Construct: shRNA TRCN0000039723

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005188.1-2901s1c1
DNA Barcode:
CCAGTGAGTTGGGAGTTATTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CBL (867)
Gene Description:
Cbl proto-oncogene
Transcript:
RefSeq NM_005188.1 (NON-CURRENT)
Match location:
Position 2901 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000039723)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGTGAGTTGGGAGTTATTA
Hairpin Sequence:
5'-CCGG-CCAGTGAGTTGGGAGTTATTA-CTCGAG-TAATAACTCCCAACTCACTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGTGAGTTGGGAGTTATTACTCGAGTAATAACTCCCAACTCACTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGTGAGTTGGGAGTTATTACTCGAGTAATAACTCCCAACTCACTGG-3'

Other clones with same target sequence:

  1. TRCN0000288695