Construct: shRNA TRCN0000039738

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001363.2-2024s1c1
DNA Barcode:
GCTCAGTGAAATGCTGTAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DKC1 (1736)
Gene Description:
dyskerin pseudouridine synthase 1
Transcript:
RefSeq NM_001363.2 (NON-CURRENT)
Match location:
Position 2024 (3UTR)
CRISPR Mechanism
CRISPR

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000039738)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCAGTGAAATGCTGTAGAA
Hairpin Sequence:
5'-CCGG-GCTCAGTGAAATGCTGTAGAA-CTCGAG-TTCTACAGCATTTCACTGAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCAGTGAAATGCTGTAGAACTCGAGTTCTACAGCATTTCACTGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCAGTGAAATGCTGTAGAACTCGAGTTCTACAGCATTTCACTGAGC-3'

Other clones with same target sequence:

  1. TRCN0000332894