Construct: shRNA TRCN0000039864

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002382.3-370s1c1
DNA Barcode:
CCACAGAATATATCCAGTATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAX (4149)
Gene Description:
MYC associated factor X
Transcript:
RefSeq NM_002382.3 (NON-CURRENT)
Match location:
Position 370 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000039864)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACAGAATATATCCAGTATA
Hairpin Sequence:
5'-CCGG-CCACAGAATATATCCAGTATA-CTCGAG-TATACTGGATATATTCTGTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACAGAATATATCCAGTATACTCGAGTATACTGGATATATTCTGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACAGAATATATCCAGTATACTCGAGTATACTGGATATATTCTGTGG-3'

Other clones with same target sequence:

  1. TRCN0000231549