Construct: shRNA TRCN0000039875

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001658.2-435s1c1
DNA Barcode:
CCTCCTGGTGTTCGCCAACAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ARF1 (375)
Gene Description:
ADP ribosylation factor 1
Transcript:
RefSeq NM_001658.2 (NON-CURRENT)
Match location:
Position 435 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000039875)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCCTGGTGTTCGCCAACAA
Hairpin Sequence:
5'-CCGG-CCTCCTGGTGTTCGCCAACAA-CTCGAG-TTGTTGGCGAACACCAGGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCCTGGTGTTCGCCAACAACTCGAGTTGTTGGCGAACACCAGGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCCTGGTGTTCGCCAACAACTCGAGTTGTTGGCGAACACCAGGAGG-3'

Other clones with same target sequence:

  1. TRCN0000288584