Construct: shRNA TRCN0000039877

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001658.2-510s1c1
DNA Barcode:
GCACTCACTACGCCACAGGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ARF1 (375)
Gene Description:
ADP ribosylation factor 1
Transcript:
RefSeq NM_001658.2 (NON-CURRENT)
Match location:
Position 510 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000039877)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACTCACTACGCCACAGGAA
Hairpin Sequence:
5'-CCGG-GCACTCACTACGCCACAGGAA-CTCGAG-TTCCTGTGGCGTAGTGAGTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACTCACTACGCCACAGGAACTCGAGTTCCTGTGGCGTAGTGAGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACTCACTACGCCACAGGAACTCGAGTTCCTGTGGCGTAGTGAGTGC-3'

Other clones with same target sequence:

  1. TRCN0000288645