Construct: shRNA TRCN0000039896

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005541.2-2387s1c1
DNA Barcode:
GCAGAAGGTCTTCCTACACTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
INPP5D (3635)
Gene Description:
inositol polyphosphate-5-phosphatase D
Transcript:
RefSeq NM_005541.2 (NON-CURRENT)
Match location:
Position 2387 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000039896)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGAAGGTCTTCCTACACTT
Hairpin Sequence:
5'-CCGG-GCAGAAGGTCTTCCTACACTT-CTCGAG-AAGTGTAGGAAGACCTTCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGAAGGTCTTCCTACACTTCTCGAGAAGTGTAGGAAGACCTTCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGAAGGTCTTCCTACACTTCTCGAGAAGTGTAGGAAGACCTTCTGC-3'

Other clones with same target sequence:

(none)