Construct: shRNA TRCN0000039930

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004064.2-636s1c1
DNA Barcode:
GCGCAAGTGGAATTTCGATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CDKN1B (1027)
Gene Description:
cyclin dependent kinase inhibitor 1B
Transcript:
RefSeq NM_004064.2 (NON-CURRENT)
Match location:
Position 636 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000039930)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGCAAGTGGAATTTCGATTT
Hairpin Sequence:
5'-CCGG-GCGCAAGTGGAATTTCGATTT-CTCGAG-AAATCGAAATTCCACTTGCGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGCAAGTGGAATTTCGATTTCTCGAGAAATCGAAATTCCACTTGCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGCAAGTGGAATTTCGATTTCTCGAGAAATCGAAATTCCACTTGCGC-3'

Other clones with same target sequence:

  1. TRCN0000288586