Construct: shRNA TRCN0000039933

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006244.2-2642s1c1
DNA Barcode:
GTTCACAGTAATCATGGTCTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PPP2R5B (5526)
Gene Description:
protein phosphatase 2 regulatory subunit B'beta
Transcript:
RefSeq NM_006244.2 (NON-CURRENT)
Match location:
Position 2642 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000039933)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTTCACAGTAATCATGGTCTA
Hairpin Sequence:
5'-CCGG-GTTCACAGTAATCATGGTCTA-CTCGAG-TAGACCATGATTACTGTGAAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTTCACAGTAATCATGGTCTACTCGAGTAGACCATGATTACTGTGAACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTTCACAGTAATCATGGTCTACTCGAGTAGACCATGATTACTGTGAAC-3'

Other clones with same target sequence:

(none)