Construct: shRNA TRCN0000039937

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006244.2-1023s1c1
DNA Barcode:
CCCTGAATTTGACCCTGAAGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PPP2R5B (5526)
Gene Description:
protein phosphatase 2 regulatory subunit B'beta
Transcript:
RefSeq NM_006244.2 (NON-CURRENT)
Match location:
Position 1023 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000039937)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTGAATTTGACCCTGAAGA
Hairpin Sequence:
5'-CCGG-CCCTGAATTTGACCCTGAAGA-CTCGAG-TCTTCAGGGTCAAATTCAGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTGAATTTGACCCTGAAGACTCGAGTCTTCAGGGTCAAATTCAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTGAATTTGACCCTGAAGACTCGAGTCTTCAGGGTCAAATTCAGGG-3'

Other clones with same target sequence:

(none)