Construct: shRNA TRCN0000039956

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025259.3-577s1c1
DNA Barcode:
CCTCTCTTCCATTATTCCCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MSH5 (4439)
Gene Description:
mutS homolog 5
Transcript:
RefSeq NM_025259.3 (NON-CURRENT)
Match location:
Position 577 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000039956)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCTCTTCCATTATTCCCTT
Hairpin Sequence:
5'-CCGG-CCTCTCTTCCATTATTCCCTT-CTCGAG-AAGGGAATAATGGAAGAGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCTCTTCCATTATTCCCTTCTCGAGAAGGGAATAATGGAAGAGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCTCTTCCATTATTCCCTTCTCGAGAAGGGAATAATGGAAGAGAGG-3'

Other clones with same target sequence:

(none)