Construct: shRNA TRCN0000039970

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001626.2-972s1c1
DNA Barcode:
GCAGAGATTGTCTCGGCTCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
AKT2 (208)
Gene Description:
AKT serine/threonine kinase 2
Transcript:
RefSeq NM_001626.2 (NON-CURRENT)
Match location:
Position 972 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000039970)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGAGATTGTCTCGGCTCTT
Hairpin Sequence:
5'-CCGG-GCAGAGATTGTCTCGGCTCTT-CTCGAG-AAGAGCCGAGACAATCTCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGAGATTGTCTCGGCTCTTCTCGAGAAGAGCCGAGACAATCTCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGAGATTGTCTCGGCTCTTCTCGAGAAGAGCCGAGACAATCTCTGC-3'

Other clones with same target sequence:

  1. TRCN0000174056