Construct: shRNA TRCN0000039995

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000127.1-1454s1c1
DNA Barcode:
CCCAACTTTGATGTTTCTATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
EXT1 (2131)
Gene Description:
exostosin glycosyltransferase 1
Transcript:
RefSeq NM_000127.1 (NON-CURRENT)
Match location:
Position 1454 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000039995)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAACTTTGATGTTTCTATT
Hairpin Sequence:
5'-CCGG-CCCAACTTTGATGTTTCTATT-CTCGAG-AATAGAAACATCAAAGTTGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAACTTTGATGTTTCTATTCTCGAGAATAGAAACATCAAAGTTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAACTTTGATGTTTCTATTCTCGAGAATAGAAACATCAAAGTTGGG-3'

Other clones with same target sequence:

(none)