Construct: shRNA TRCN0000039998

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002093.2-924s1c1
DNA Barcode:
CCACTGATTATACCTCTAGTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GSK3B (2932)
Gene Description:
glycogen synthase kinase 3 beta
Transcript:
RefSeq NM_002093.2 (NON-CURRENT)
Match location:
Position 924 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000039998)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACTGATTATACCTCTAGTA
Hairpin Sequence:
5'-CCGG-CCACTGATTATACCTCTAGTA-CTCGAG-TACTAGAGGTATAATCAGTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACTGATTATACCTCTAGTACTCGAGTACTAGAGGTATAATCAGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACTGATTATACCTCTAGTACTCGAGTACTAGAGGTATAATCAGTGG-3'

Other clones with same target sequence:

(none)