Construct: shRNA TRCN0000040035

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005901.3-1631s1c1
DNA Barcode:
GCGTTGCTCAAGCATGTCATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SMAD2 (4087)
Gene Description:
SMAD family member 2
Transcript:
RefSeq NM_005901.3 (NON-CURRENT)
Match location:
Position 1631 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000040035)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGTTGCTCAAGCATGTCATA
Hairpin Sequence:
5'-CCGG-GCGTTGCTCAAGCATGTCATA-CTCGAG-TATGACATGCTTGAGCAACGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGTTGCTCAAGCATGTCATACTCGAGTATGACATGCTTGAGCAACGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGTTGCTCAAGCATGTCATACTCGAGTATGACATGCTTGAGCAACGC-3'

Other clones with same target sequence:

  1. TRCN0000288592