Construct: shRNA TRCN0000040069

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000633.1-64s1c1
DNA Barcode:
CCGGGAGATAGTGATGAAGTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BCL2 (596)
Gene Description:
BCL2 apoptosis regulator
Transcript:
RefSeq NM_000633.1 (NON-CURRENT)
Match location:
Position 64 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000040069)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGGGAGATAGTGATGAAGTA
Hairpin Sequence:
5'-CCGG-CCGGGAGATAGTGATGAAGTA-CTCGAG-TACTTCATCACTATCTCCCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGGGAGATAGTGATGAAGTACTCGAGTACTTCATCACTATCTCCCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGGGAGATAGTGATGAAGTACTCGAGTACTTCATCACTATCTCCCGG-3'

Other clones with same target sequence:

(none)