Construct: shRNA TRCN0000040093

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000038.2-9031s1c1
DNA Barcode:
GCTGTGAAATTCACAGTAATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
APC (324)
Gene Description:
APC regulator of WNT signaling pathway
Transcript:
RefSeq NM_000038.2 (NON-CURRENT)
Match location:
Position 9031 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000040093)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGTGAAATTCACAGTAATA
Hairpin Sequence:
5'-CCGG-GCTGTGAAATTCACAGTAATA-CTCGAG-TATTACTGTGAATTTCACAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGTGAAATTCACAGTAATACTCGAGTATTACTGTGAATTTCACAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGTGAAATTCACAGTAATACTCGAGTATTACTGTGAATTTCACAGC-3'

Other clones with same target sequence:

(none)