Construct: shRNA TRCN0000040123

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000389.2-640s1c1
DNA Barcode:
CGCTCTACATCTTCTGCCTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CDKN1A (1026)
Gene Description:
cyclin dependent kinase inhibitor 1A
Transcript:
RefSeq NM_000389.2 (NON-CURRENT)
Match location:
Position 640 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000040123)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCTCTACATCTTCTGCCTTA
Hairpin Sequence:
5'-CCGG-CGCTCTACATCTTCTGCCTTA-CTCGAG-TAAGGCAGAAGATGTAGAGCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCTCTACATCTTCTGCCTTACTCGAGTAAGGCAGAAGATGTAGAGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCTCTACATCTTCTGCCTTACTCGAGTAAGGCAGAAGATGTAGAGCG-3'

Other clones with same target sequence:

  1. TRCN0000287021