Construct: shRNA TRCN0000040163

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000321.1-4075s1c1
DNA Barcode:
CCACATTATTTCTAGTCCAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RB1 (5925)
Gene Description:
RB transcriptional corepressor 1
Transcript:
RefSeq NM_000321.1 (NON-CURRENT)
Match location:
Position 4075 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000040163)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACATTATTTCTAGTCCAAA
Hairpin Sequence:
5'-CCGG-CCACATTATTTCTAGTCCAAA-CTCGAG-TTTGGACTAGAAATAATGTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACATTATTTCTAGTCCAAACTCGAGTTTGGACTAGAAATAATGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACATTATTTCTAGTCCAAACTCGAGTTTGGACTAGAAATAATGTGG-3'

Other clones with same target sequence:

  1. TRCN0000288710