Construct: shRNA TRCN0000040186

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003747.1-834s1c1
DNA Barcode:
GCCAGGGATAACTGGAACTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TNKS (8658)
Gene Description:
tankyrase
Transcript:
RefSeq NM_003747.1 (NON-CURRENT)
Match location:
Position 834 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000040186)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAGGGATAACTGGAACTAT
Hairpin Sequence:
5'-CCGG-GCCAGGGATAACTGGAACTAT-CTCGAG-ATAGTTCCAGTTATCCCTGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAGGGATAACTGGAACTATCTCGAGATAGTTCCAGTTATCCCTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAGGGATAACTGGAACTATCTCGAGATAGTTCCAGTTATCCCTGGC-3'

Other clones with same target sequence:

  1. TRCN0000333000