Construct: shRNA TRCN0000040196

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000059.1-1832s1c1
DNA Barcode:
CCTCTGAAAGTGGACTGGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BRCA2 (675)
Gene Description:
BRCA2 DNA repair associated
Transcript:
RefSeq NM_000059.1 (NON-CURRENT)
Match location:
Position 1832 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000040196)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCTGAAAGTGGACTGGAAA
Hairpin Sequence:
5'-CCGG-CCTCTGAAAGTGGACTGGAAA-CTCGAG-TTTCCAGTCCACTTTCAGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCTGAAAGTGGACTGGAAACTCGAGTTTCCAGTCCACTTTCAGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCTGAAAGTGGACTGGAAACTCGAGTTTCCAGTCCACTTTCAGAGG-3'

Other clones with same target sequence:

(none)