Construct: shRNA TRCN0000040201

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133259.2-2443s1c1
DNA Barcode:
CGCAGCTTTAAGAGGTGAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LRPPRC (10128)
Gene Description:
leucine rich pentatricopeptide repeat containing
Transcript:
RefSeq NM_133259.2 (NON-CURRENT)
Match location:
Position 2443 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000040201)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCAGCTTTAAGAGGTGAAAT
Hairpin Sequence:
5'-CCGG-CGCAGCTTTAAGAGGTGAAAT-CTCGAG-ATTTCACCTCTTAAAGCTGCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCAGCTTTAAGAGGTGAAATCTCGAGATTTCACCTCTTAAAGCTGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCAGCTTTAAGAGGTGAAATCTCGAGATTTCACCTCTTAAAGCTGCG-3'

Other clones with same target sequence:

  1. TRCN0000288680