Construct: shRNA TRCN0000040252

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_198283.1-493s1c1
DNA Barcode:
GCATCCACAACCCTCATCATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
EYS (346007)
Gene Description:
eyes shut homolog
Transcript:
RefSeq NM_198283.1 (CURRENT)
Match location:
Position 493 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000040252)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATCCACAACCCTCATCATA
Hairpin Sequence:
5'-CCGG-GCATCCACAACCCTCATCATA-CTCGAG-TATGATGAGGGTTGTGGATGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATCCACAACCCTCATCATACTCGAGTATGATGAGGGTTGTGGATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATCCACAACCCTCATCATACTCGAGTATGATGAGGGTTGTGGATGC-3'

Other clones with same target sequence:

(none)