Construct: shRNA TRCN0000040284

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015378.1-1577s1c1
DNA Barcode:
GCCTCGTGTATGAACACGTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
VPS13D (55187)
Gene Description:
vacuolar protein sorting 13 homolog D
Transcript:
RefSeq NM_015378.1 (NON-CURRENT)
Match location:
Position 1577 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000040284)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTCGTGTATGAACACGTAT
Hairpin Sequence:
5'-CCGG-GCCTCGTGTATGAACACGTAT-CTCGAG-ATACGTGTTCATACACGAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTCGTGTATGAACACGTATCTCGAGATACGTGTTCATACACGAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTCGTGTATGAACACGTATCTCGAGATACGTGTTCATACACGAGGC-3'

Other clones with same target sequence:

(none)