Construct: shRNA TRCN0000040490

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_023270.3-1058s1c1
DNA Barcode:
CGCATCCTAACCTGCAATCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rnf128 (66889)
Gene Description:
ring finger protein 128
Transcript:
RefSeq NM_023270.3 (NON-CURRENT)
Match location:
Position 1058 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000040490)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCATCCTAACCTGCAATCAT
Hairpin Sequence:
5'-CCGG-CGCATCCTAACCTGCAATCAT-CTCGAG-ATGATTGCAGGTTAGGATGCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCATCCTAACCTGCAATCATCTCGAGATGATTGCAGGTTAGGATGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCATCCTAACCTGCAATCATCTCGAGATGATTGCAGGTTAGGATGCG-3'

Other clones with same target sequence:

(none)