Construct: shRNA TRCN0000040500

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019748.1-784s1c1
DNA Barcode:
GCTGAAATTCCGCACAGATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sae1 (56459)
Gene Description:
SUMO1 activating enzyme subunit 1
Transcript:
RefSeq NM_019748.1 (NON-CURRENT)
Match location:
Position 784 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000040500)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGAAATTCCGCACAGATAA
Hairpin Sequence:
5'-CCGG-GCTGAAATTCCGCACAGATAA-CTCGAG-TTATCTGTGCGGAATTTCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGAAATTCCGCACAGATAACTCGAGTTATCTGTGCGGAATTTCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGAAATTCCGCACAGATAACTCGAGTTATCTGTGCGGAATTTCAGC-3'

Other clones with same target sequence:

  1. TRCN0000301271