Construct: shRNA TRCN0000040574

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_029438.2-367s1c1
DNA Barcode:
GCCAAGAACCTTGCAAAGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Smurf1 (75788)
Gene Description:
SMAD specific E3 ubiquitin protein ligase 1
Transcript:
RefSeq NM_029438.2 (NON-CURRENT)
Match location:
Position 367 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000040574)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAAGAACCTTGCAAAGAAA
Hairpin Sequence:
5'-CCGG-GCCAAGAACCTTGCAAAGAAA-CTCGAG-TTTCTTTGCAAGGTTCTTGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAAGAACCTTGCAAAGAAACTCGAGTTTCTTTGCAAGGTTCTTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAAGAACCTTGCAAAGAAACTCGAGTTTCTTTGCAAGGTTCTTGGC-3'

Other clones with same target sequence:

(none)