Construct: shRNA TRCN0000040593

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011278.1-812s1c1
DNA Barcode:
CCCTTAAGAATGCTAACACTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rnf4 (19822)
Gene Description:
ring finger protein 4
Transcript:
RefSeq NM_011278.1 (NON-CURRENT)
Match location:
Position 812 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000040593)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTTAAGAATGCTAACACTT
Hairpin Sequence:
5'-CCGG-CCCTTAAGAATGCTAACACTT-CTCGAG-AAGTGTTAGCATTCTTAAGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTTAAGAATGCTAACACTTCTCGAGAAGTGTTAGCATTCTTAAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTTAAGAATGCTAACACTTCTCGAGAAGTGTTAGCATTCTTAAGGG-3'

Other clones with same target sequence:

  1. TRCN0000323985