Construct: shRNA TRCN0000040652

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_356817.1-352s1c1
DNA Barcode:
CGCCGTCAGTTGCTCTGTGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
LOC383042 (383042)
Gene Description:
similar to ubiquitin-conjugating enzyme HBUCE1
Transcript:
RefSeq XM_356817.1 (NON-CURRENT)
Match location:
Position 352 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000040652)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCCGTCAGTTGCTCTGTGAA
Hairpin Sequence:
5'-CCGG-CGCCGTCAGTTGCTCTGTGAA-CTCGAG-TTCACAGAGCAACTGACGGCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCCGTCAGTTGCTCTGTGAACTCGAGTTCACAGAGCAACTGACGGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCCGTCAGTTGCTCTGTGAACTCGAGTTCACAGAGCAACTGACGGCG-3'

Other clones with same target sequence:

(none)