Construct: shRNA TRCN0000040671
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- XM_357023.1-865s1c1
- DNA Barcode:
- ACTGTGGAAATGGGCTATGAA
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- LOC383385 (383385)
- Gene Description:
- similar to ubiquitin-protein ligase NEDD4-like; neural precursor cell expressed, developmentally down-regulated gene 4-like; potential epithelial sodium channel regulator; homolog of yeast ubiquitin-protein ligase Rsp5
- Transcript:
- RefSeq XM_357023.1 (NON-CURRENT)
- Match location:
- Position 865 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000040671)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- ACTGTGGAAATGGGCTATGAA
- Hairpin Sequence:
-
5'-CCGG-ACTGTGGAAATGGGCTATGAA-CTCGAG-TTCATAGCCCATTTCCACAGT-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGACTGTGGAAATGGGCTATGAACTCGAGTTCATAGCCCATTTCCACAGTTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAACTGTGGAAATGGGCTATGAACTCGAGTTCATAGCCCATTTCCACAGT-3'
Other clones with same target sequence:
(none)
