Construct: shRNA TRCN0000040753

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024288.1-2115s1c1
DNA Barcode:
CCTGCATTTGAGTTCTGTCTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rmnd5a (68477)
Gene Description:
required for meiotic nuclear division 5 homolog A
Transcript:
RefSeq NM_024288.1 (NON-CURRENT)
Match location:
Position 2115 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000040753)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGCATTTGAGTTCTGTCTA
Hairpin Sequence:
5'-CCGG-CCTGCATTTGAGTTCTGTCTA-CTCGAG-TAGACAGAACTCAAATGCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGCATTTGAGTTCTGTCTACTCGAGTAGACAGAACTCAAATGCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGCATTTGAGTTCTGTCTACTCGAGTAGACAGAACTCAAATGCAGG-3'

Other clones with same target sequence:

(none)