Construct: shRNA TRCN0000040757

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024288.1-790s1c1
DNA Barcode:
CCCAAGTCAGAAAGAACCATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rmnd5a (68477)
Gene Description:
required for meiotic nuclear division 5 homolog A
Transcript:
RefSeq NM_024288.1 (NON-CURRENT)
Match location:
Position 790 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000040757)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAAGTCAGAAAGAACCATT
Hairpin Sequence:
5'-CCGG-CCCAAGTCAGAAAGAACCATT-CTCGAG-AATGGTTCTTTCTGACTTGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAAGTCAGAAAGAACCATTCTCGAGAATGGTTCTTTCTGACTTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAAGTCAGAAAGAACCATTCTCGAGAATGGTTCTTTCTGACTTGGG-3'

Other clones with same target sequence:

  1. TRCN0000335328