Construct: shRNA TRCN0000040777

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172442.2-1885s1c1
DNA Barcode:
CGCCGTCTCATCTTTGCCATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dtx4 (207521)
Gene Description:
deltex 4, E3 ubiquitin ligase
Transcript:
RefSeq NM_172442.2 (NON-CURRENT)
Match location:
Position 1885 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000040777)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCCGTCTCATCTTTGCCATT
Hairpin Sequence:
5'-CCGG-CGCCGTCTCATCTTTGCCATT-CTCGAG-AATGGCAAAGATGAGACGGCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCCGTCTCATCTTTGCCATTCTCGAGAATGGCAAAGATGAGACGGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCCGTCTCATCTTTGCCATTCTCGAGAATGGCAAAGATGAGACGGCG-3'

Other clones with same target sequence:

(none)