Construct: shRNA TRCN0000040826

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009456.1-324s1c1
DNA Barcode:
CCACCAAGACTGACCAAGTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ube2l3 (22195)
Gene Description:
ubiquitin-conjugating enzyme E2L 3
Transcript:
RefSeq NM_009456.1 (NON-CURRENT)
Match location:
Position 324 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000040826)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACCAAGACTGACCAAGTAA
Hairpin Sequence:
5'-CCGG-CCACCAAGACTGACCAAGTAA-CTCGAG-TTACTTGGTCAGTCTTGGTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACCAAGACTGACCAAGTAACTCGAGTTACTTGGTCAGTCTTGGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACCAAGACTGACCAAGTAACTCGAGTTACTTGGTCAGTCTTGGTGG-3'

Other clones with same target sequence:

  1. TRCN0000317880