Construct: shRNA TRCN0000040893

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145420.1-1156s1c1
DNA Barcode:
GCACACTGTATTTCTCAGTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ube2d1 (216080)
Gene Description:
ubiquitin-conjugating enzyme E2D 1
Transcript:
RefSeq NM_145420.1 (NON-CURRENT)
Match location:
Position 1156 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000040893)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACACTGTATTTCTCAGTAT
Hairpin Sequence:
5'-CCGG-GCACACTGTATTTCTCAGTAT-CTCGAG-ATACTGAGAAATACAGTGTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACACTGTATTTCTCAGTATCTCGAGATACTGAGAAATACAGTGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACACTGTATTTCTCAGTATCTCGAGATACTGAGAAATACAGTGTGC-3'

Other clones with same target sequence:

  1. TRCN0000316952