Construct: shRNA TRCN0000040932

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_080510.1-666s1c1
DNA Barcode:
CGTTTCTTTACGGAGGAGCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Trim69 (70928)
Gene Description:
tripartite motif-containing 69
Transcript:
RefSeq NM_080510.1 (NON-CURRENT)
Match location:
Position 666 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000040932)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTTTCTTTACGGAGGAGCTT
Hairpin Sequence:
5'-CCGG-CGTTTCTTTACGGAGGAGCTT-CTCGAG-AAGCTCCTCCGTAAAGAAACG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTTTCTTTACGGAGGAGCTTCTCGAGAAGCTCCTCCGTAAAGAAACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTTTCTTTACGGAGGAGCTTCTCGAGAAGCTCCTCCGTAAAGAAACG-3'

Other clones with same target sequence:

(none)