Construct: shRNA TRCN0000040933

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153789.2-866s1c1
DNA Barcode:
CCCTATTAACAGGATAGCTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mylip (218203)
Gene Description:
myosin regulatory light chain interacting protein
Transcript:
RefSeq NM_153789.2 (NON-CURRENT)
Match location:
Position 866 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000040933)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTATTAACAGGATAGCTTA
Hairpin Sequence:
5'-CCGG-CCCTATTAACAGGATAGCTTA-CTCGAG-TAAGCTATCCTGTTAATAGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTATTAACAGGATAGCTTACTCGAGTAAGCTATCCTGTTAATAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTATTAACAGGATAGCTTACTCGAGTAAGCTATCCTGTTAATAGGG-3'

Other clones with same target sequence:

(none)