Construct: shRNA TRCN0000040941

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009543.1-1718s1c1
DNA Barcode:
GCTCATTACAACCTGAAGTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rnf103 (22644)
Gene Description:
ring finger protein 103
Transcript:
RefSeq NM_009543.1 (NON-CURRENT)
Match location:
Position 1718 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000040941)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCATTACAACCTGAAGTAA
Hairpin Sequence:
5'-CCGG-GCTCATTACAACCTGAAGTAA-CTCGAG-TTACTTCAGGTTGTAATGAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCATTACAACCTGAAGTAACTCGAGTTACTTCAGGTTGTAATGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCATTACAACCTGAAGTAACTCGAGTTACTTCAGGTTGTAATGAGC-3'

Other clones with same target sequence:

(none)