Construct: shRNA TRCN0000041001

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175201.2-1616s1c1
DNA Barcode:
GCTTCCTTCGTACAGGTTCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rnf38 (73469)
Gene Description:
ring finger protein 38
Transcript:
RefSeq NM_175201.2 (NON-CURRENT)
Match location:
Position 1616 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000041001)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTCCTTCGTACAGGTTCAA
Hairpin Sequence:
5'-CCGG-GCTTCCTTCGTACAGGTTCAA-CTCGAG-TTGAACCTGTACGAAGGAAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTCCTTCGTACAGGTTCAACTCGAGTTGAACCTGTACGAAGGAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTCCTTCGTACAGGTTCAACTCGAGTTGAACCTGTACGAAGGAAGC-3'

Other clones with same target sequence:

  1. TRCN0000302812