Construct: shRNA TRCN0000041013

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028705.2-1186s1c1
DNA Barcode:
GCTTTAAGTATCGTGTCGTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Herc3 (73998)
Gene Description:
hect domain and RLD 3
Transcript:
RefSeq NM_028705.2 (NON-CURRENT)
Match location:
Position 1186 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000041013)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTTAAGTATCGTGTCGTTA
Hairpin Sequence:
5'-CCGG-GCTTTAAGTATCGTGTCGTTA-CTCGAG-TAACGACACGATACTTAAAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTTAAGTATCGTGTCGTTACTCGAGTAACGACACGATACTTAAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTTAAGTATCGTGTCGTTACTCGAGTAACGACACGATACTTAAAGC-3'

Other clones with same target sequence:

(none)