Construct: shRNA TRCN0000041031

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028717.2-4782s1c1
DNA Barcode:
GCAGCAGATCAGCACAACATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Als2 (74018)
Gene Description:
amyotrophic lateral sclerosis 2 (juvenile)
Transcript:
RefSeq NM_028717.2 (NON-CURRENT)
Match location:
Position 4782 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000041031)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGCAGATCAGCACAACATT
Hairpin Sequence:
5'-CCGG-GCAGCAGATCAGCACAACATT-CTCGAG-AATGTTGTGCTGATCTGCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGCAGATCAGCACAACATTCTCGAGAATGTTGTGCTGATCTGCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGCAGATCAGCACAACATTCTCGAGAATGTTGTGCTGATCTGCTGC-3'

Other clones with same target sequence:

  1. TRCN0000316885