Construct: shRNA TRCN0000041050

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172281.1-1486s1c1
DNA Barcode:
GCGCATCGAGTTTGAACAGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rnf40 (233900)
Gene Description:
ring finger protein 40
Transcript:
RefSeq NM_172281.1 (NON-CURRENT)
Match location:
Position 1486 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000041050)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGCATCGAGTTTGAACAGAA
Hairpin Sequence:
5'-CCGG-GCGCATCGAGTTTGAACAGAA-CTCGAG-TTCTGTTCAAACTCGATGCGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGCATCGAGTTTGAACAGAACTCGAGTTCTGTTCAAACTCGATGCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGCATCGAGTTTGAACAGAACTCGAGTTCTGTTCAAACTCGATGCGC-3'

Other clones with same target sequence:

(none)